Biquinoline Series: Multifunctional Reagents from Coordination Chemistry to Protein Quantification - Bharuch

In coordination chemistry, analytical detection, and biochemistry, 2,2'-Biquinoline CAS:119-91-5 and its derivatives form a complementary reagent family. From metal coordination to copper detection and protein quantification, these compounds play diverse roles in the laboratory.

2,2'-Biquinoline: Classic Bidentate Ligand and Copper Reagent
2,2'-Biquinoline (also known as cuproine) is a white to pale yellow crystalline powder with a melting point of approximately 192-195°C. Its rigid planar structure consists of two quinoline units linked at the 2-position, with nitrogen atoms that coordinate metal centers synergistically to form stable chelates.

In analytical chemistry, 2,2'-biquinoline is a classic photometric reagent for copper, forming a colored complex with Cu(I). Studies show this complex can be adsorbed on activated carbon for trace copper preconcentration, achieving a detection limit as low as 0.33 ng/mL and successfully applied to copper determination in seawater. In materials science, it serves as a ligand for synthesizing heteroleptic Cu(I) complexes used in light-emitting diodes and phosphorescent dyes for live-cell imaging.

2,2'-Biquinoline-4,4'-dicarboxylic Acid: MOF Building Unit
2,2'-Biquinoline-4,4'-dicarboxylic acid (CAS 1245-13-2) introduces two carboxylic acid groups onto the biquinoline skeleton, combining coordinating nitrogen atoms with carboxylic oxygen atoms for dual coordination capability. This compound has been used to synthesize and characterize six metal-organic coordination polymers, making it an ideal ligand precursor for functional MOF materials.

Bicinchoninic Acid Disodium Salt: Core Reagent for BCA Protein Assay
Bicinchoninic acid disodium salt (BCA, CAS 979-88-4) is one of the most widely used members of the biquinoline family. Its core application is as the chromogenic reagent in the BCA protein quantification assay.

The method is based on the reduction of Cu2+ to Cu+ by proteins under alkaline conditions, followed by two BCA molecules chelating Cu+ to form a stable purple complex with maximum absorbance at 562 nm. This colorimetric assay typically works in the range of 20-2000 μg/mL and is a standard method for protein concentration determination in biochemistry laboratories.

Why Choose UCHEM?
UCHEM maintains stock of all three biquinoline series products: 2,2'-Biquinoline (CAS 119-91-5), 2,2'-Biquinoline-4,4'-dicarboxylic acid (CAS 1245-13-2), and Bicinchoninic acid disodium salt (CAS 979-88-4). Strict quality control ensures batch consistency, supporting needs from R&D to bulk production. Contact us for technical specifications and pricing.