ISO/IEC 17025 accreditation is a globally used standard for calibration and testing laboratories. The ISO 17025:2017 audit checklist is a document required to verify the accreditation system and ensure the implementation of a laboratory management system. The checklist contains over 200 audit questions for the department and clause-wise requirements, helping organizations achieve accreditation quickly. It is a valuable tool for auditors to create audit questionnaires and verify the effectiveness of their laboratory management system.